Active Recombinant Human ADAM12, His-tagged

• Cat.No.: ADAM12-233H
• Product Overview: Recombinant Human ADAM12 (Accession # AAC08702) Arg29-Ser513, fused with an N-terminal signal peptide and a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: His
• Predicted N Terminal: Arg29
• Form: Supplied as a 0.2 µm filtered solution in HEPES, NaCl and CaCl2.
• Bio-activity: Measured by its ability to cleave IGFBP-3.
• Molecular Mass: Recombinant Human ADAM12, His-tagged has a calculated MW of 55 kDa (Pro) & 34 kDa (Mature). In SDS-PAGE migrates as 28 kDa, 44 kDa & 68 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ADAM12 ADAM metallopeptidase domain 12 [ Homo sapiens ]
• Official Symbol: ADAM12
• Synonyms: ADAM12; ADAM metallopeptidase domain 12; a disintegrin and metalloproteinase domain 12 (meltrin alpha); disintegrin and metalloproteinase domain-containing protein 12; MCMPMltna; meltrin alpha; MLTN; meltrin-alpha; MCMP; MLTNA
• Gene ID: 8038
• mRNA Refseq: NM_003474
• Protein Refseq: NP_003465
• MIM: 602714
• UniProt ID: O43184
• Chromosome Location: 10q26

Reviews

08/22/2020

The technical staff evaluated my experimental process and results and made many constructive comments

05/28/2019

Protein products are put into experiments with good effect and reliable experimental results

Q&As

In addition to studies related to breast cancer, what other diseases is ADAD12 currently being used to study?

Currently, ADAM12 is also involved in the study of lung cancer and liver cancer.

Does ADAM12 affect Vimentin expression?

ADAM12 upregulates the expression of Vimentin.

Does ADAM12 interact with insulin-like growth factor-binding protein-3 (IGFBP)-3?

ADAM12 interacts with insulin-like growth factor binding protein(IGFBP)-3.

I want to buy ADAM12-related proteins, do you provide other proteins?

We offer myoferlin and Vimentin proteins in addition to ADAM12 proteins.

What protein does ADAM12 hydrolyze?

ADAM12 protein can hydrolyze Delta-like 1、Ephrin-A1, etc.

What are the two conformations of ADAM12?

The two conformations of ADAM are the membrane-penetrating full-length type (ADAM12-L) and the secretory type (ADAM12-S), respectively.