Active Recombinant Human ALK(1058-end) Protein, N-His-tagged

• Cat.No.: ALK-18H
• Product Overview: N-terminal 6His tagged, recombinant, human ALK, amino acids 1058–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: ALK or anaplastic lymphoma kinase (Ki-1) is a membrane-spanning tyrosine kinase receptor that is most closely related to leukocyte tyrosine kinase. No function has been ascribed for ALK; however, its restricted expression in neural tissue suggests that this receptor might play an important role in the normal development and function of the nervous system. Large-cell anaplastic lymphoma is a subtype of non-Hodgkin's lymphoma. Over half of these lymphomas have a chromosomal translocation t(2;5), that leads to the expression of a hybrid protein comprised of the nucleolar phosphoprotein nucleophosmin (NPM) and ALK.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1058-end
• Form: 0.51mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 254U/mg, where one unit of ALK activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63.8 kDa
• AA Sequence: 1 MHHHHHHEFV YRRKHQELQA MQMELQSPEY KLSKLRTSTI MTDYNPNYCF AGKTSSISDL 61 KEVPRKNITL IRGLGHGAFG EVYEGQVSGM PNDPSPLQVA VKTLPEVCSE QDELDFLMEA 121 LIISKFNHQN IVRCIGVSLQ SLPRFILLEL MAGGDLKSFL RETRPRPSQP SSLAMLDLLH 181 VARDIACGCQ YLEENHFIHR DIAARNCLLT CPGPGRVAKI GDFGMARDIY RASYYRKGGC 241 AMLPVKWMPP EAFMEGIFTS KTDTWSFGVL LWEIFSLGYM PYPSKSNQEV LEFVTSGGRM 301 DPPKNCPGPV YRIMTQCWQH QPEDRPNFAI ILERIEYCTQ DPDVINTALP IEYGPLVEEE 361 EKVPVRPKDP EGVPPLLVSQ QAKREEERSP AAPPPLPTTS SGKAAKKPTA AEVSVRVPRG 421 PAVEGGHVNM AFSQSNPPSE LHKVHGSRNK PTSLWNPTYG SWFTEKPTKK NNPIAKKEPH 481 DRGNLGLEGS CTVPPNVATG RLPGASLLLE PSSLTANMKE VPLFRLRHFP CGNVNYGYQQ 541 QGLPLEAATA PGAGHYEDTI LKSKNSMNQP GP
• Purity: Purity 59% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ALK ALK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: ALK
• Synonyms: ALK; ALK receptor tyrosine kinase; ALK1; CD246; NBLST3
• Gene ID: 238
• mRNA Refseq: NM_004304.5
• Protein Refseq: NP_004295.2
• MIM: 105590
• UniProt ID: Q9UM73

Reviews

07/05/2021

ALK has strict quality control and complies with relevant industry standards.

02/11/2021

The yield of ALK is high and has good economic benefits.

12/22/2020

The enzymatic activity of ALK reached the expected level and was able to play a role in specific reactions.

01/07/2019

Stable and reproducible results can be obtained when experiments are performed using ALK.

Q&As

How do common mutations of ALK and other genes affect the prognosis of lung cancer?

The common mutation of ALK and other genes may affect the prognosis and therapeutic effect of lung cancer, and provide new ideas for individualized treatment.

How does the phosphorylation process of ALK affect its activity?

Phosphorylation of ALK can affect its activity and signal transmission efficiency, which has an impact on the biological process of cells.

What is the application prospect of ALK in breast cancer treatment?

The application prospect of ALK in the treatment of breast cancer needs to be further studied and discussed, and the expression level in breast cancer cells is not clear.

What is the interaction of ALK with other tumor suppressor genes?

The interaction between ALK and other tumor suppressor genes may affect the occurrence and development of tumors, and provide new ideas for tumor treatment.

What role does ALK play in lung cancer signaling pathways?

This is involved in a variety of lung cancer signaling pathways, such as PI3K/Akt, MAPK/ERK, STAT3 and other pathways.

What diseases is ALK associated with?

It is associated with lung cancer, thyroid cancer, lymphoma and neuroblastoma.

Which pathway of ALK as a targeted drug?

As a targeted drug, ALK is mainly used by oral administration and intravenous injection, which can help in the treatment of diseases such as lung cancer.

What is the expression of ALK in lung cancer?

ALK is overexpressed in a small number of lung cancer patients and is involved in the occurrence and development of lung cancer.

How does ALK mutation affect the prognosis of lung cancer?

The mutation may affect the prognosis and therapeutic effect of lung cancer, suggesting the importance of ALK targeted therapy.

How the role of ALK in nervous system tumors?

ALK is involved in signal transduction and cell growth in nervous system tumors, and has a certain influence on the occurrence and development of nervous system tumors.

In what physiological processes does ALK play a role?

ALK plays an important role in many physiological processes such as neuron migration, embryonic development and osteoblast differentiation.

Can ALK be used as a screening indicator for lung cancer?

ALK can be used as one of the screening indicators for lung cancer, which can help the early diagnosis and treatment of lung cancer.

In which cell types is ALK expressed?

ALK is expressed in a variety of cell types such as the nervous system, lymphatic system, and immune system, and is involved in a variety of biological processes.

What are the regulatory mechanisms of ALK?

The regulatory mechanisms of ALK mainly include gene mutation, gene expression regulation, quality control and signal pathway regulation.

Does the prognostic impact of ALK targeted therapy on lung cancer?

The effect of ALK targeted therapy on the prognosis of lung cancer may be related to a variety of factors, including tumor stage, pathological type, treatment regimen, etc.

What is the role of ALK in the treatment of lung cancer?

ALK can be used as an important marker of targeted therapy in the treatment of lung cancer, providing an individualized treatment strategy.

Whether there is the relationship between ALK and colorectal cancer?

The relationship between ALK and colorectal cancer is unclear and needs further study.