Active Recombinant Human AMBRA1 Protein, Myc/DDK-tagged

• Cat.No.: AMBRA1-01H
• Product Overview: Purified recombinant protein of Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1) with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Description: Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
• Source: HEK293T
• Species: Human
• Tag: Myc/DDK
• Bio-activity: Binding assay
• Molecular Mass: 132.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: AMBRA1 autophagy and beclin 1 regulator 1 [ Homo sapiens (human) ]
• Official Symbol: AMBRA1
• Synonyms: AMBRA1; autophagy and beclin 1 regulator 1; DCAF3; WDR94; activating molecule in BECN1-regulated autophagy protein 1; DDB1 and CUL4 associated factor 3; WD repeat domain 94; activating molecule in beclin-1-regulated autophagy; autophagy/beclin-1 regulator 1
• Gene ID: 55626
• mRNA Refseq: NM_017749
• Protein Refseq: NP_060219
• MIM: 611359
• UniProt ID: Q9C0C7

Reviews

06/01/2019

Easy integration into multi-omics workflows for comprehensive analysis.

12/23/2018

Wide dynamic range for accurate quantification of protein changes.

11/18/2017

Low false-positive rate in target protein identification.

Q&As

What are the post-translational modifications that regulate AMBRA1 function in autophagy?

AMBRA1 undergoes post-translational modifications, including phosphorylation and ubiquitination, which regulate its interaction with other autophagy-related proteins and modulate its function in autophagy.

What role does AMBRA1 play in selective autophagy pathways, such as mitophagy and aggrephagy?

AMBRA1 participates in selective autophagy processes, such as mitophagy (selective clearance of damaged mitochondria) and aggrephagy (clearance of protein aggregates), contributing to cellular quality control mechanisms.

What is the impact of AMBRA1 on autophagy regulation?

AMBRA1 plays a critical role in autophagy regulation by promoting autophagosome formation, facilitating cargo recognition and degradation, and coordinating the activity of other autophagy-related proteins.

How does AMBRA1 influence the clearance of protein aggregates and damaged organelles through autophagy?

AMBRA1 facilitates the clearance of protein aggregates and damaged organelles through autophagy, preventing their accumulation and mitigating cellular damage.

How is AMBRA1 expression regulated during autophagy induction?

AMBRA1 expression is induced during autophagy by various signaling pathways, including nutrient deprivation, cellular stress, and growth factor signaling.

How does AMBRA1 interact with other cellular processes, such as apoptosis and cell proliferation, in coordination with autophagy?

In addition to its role in autophagy, AMBRA1 interacts with other cellular processes, such as apoptosis and cell proliferation, forming intricate crosstalk that influences cellular fate and homeostasis.

How does AMBRA1 interact with Beclin 1, ULK1, and ATG proteins?

AMBRA1 interacts with Beclin 1, ULK1, and ATG proteins through protein-protein interactions, forming complexes that regulate autophagy initiation and autophagosome formation.

What are the functional consequences of AMBRA1 depletion on autophagy activity?

Depletion of AMBRA1 leads to impaired autophagy activity, resulting in the accumulation of damaged proteins and organelles and compromised cellular homeostasis.

How does AMBRA1 contribute to autophagosome formation and maturation?

AMBRA1 promotes autophagosome formation by regulating the nucleation and elongation steps, ensuring proper assembly of the autophagy machinery and cargo sequestration.

What are the upstream signaling pathways that modulate AMBRA1 activity in autophagy?

The activity of AMBRA1 in autophagy is modulated by upstream signaling pathways, including mTORC1, AMPK, and MAPK pathways, which integrate nutrient availability and cellular stresses.