Recombinant Human AMBRA1 protein, His-tagged
Cat.No. : | AMBRA1-9609H |
Product Overview : | Recombinant Human AMBRA1 protein(906-1208 aa), fused with His tag, was expressed in E.coli. |
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Source : | E.coli |
Species : | Human |
Tag : | His |
Form : | The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole. |
Protein Length : | 906-1208 aa |
AA Sequence : | NVLYPMPADQRRHVSINSARWLPEP GLGLAYGTNKGDLVICRPEALNSGV EYYWDQLNETVFTVHSNSRSSERPG TSRATWRTDRDMGLMNAIGLQPRNP ATSVTSQGTETLALQLQNAETQTER EVPEPGTAASGPGEGEGSEYGASGE DALSRIQRLMAEGGMTAVVQREQST TMASMGGFGNNIIVSHRIHRSSQTG TEPGAAHTSSPQPSTSRGLLPEAGQ LAERGLSPRTASWDQPGTPGREPTQ PTLPSSSPVPIPVSLPSAEGPTVHC ELTNNNHLLDGGSSRGDAAGPRGEP RNR |
Purity : | 85%, by SDS-PAGE with Coomassie Brilliant Blue staining. |
Storage : | Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles. |
Reconstitution : | Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage. Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage. |
Gene Name : | AMBRA1 autophagy/beclin-1 regulator 1 [ Homo sapiens ] |
Official Symbol : | AMBRA1 |
Synonyms : | AMBRA1; autophagy/beclin-1 regulator 1; activating molecule in BECN1-regulated autophagy protein 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736; WD repeat domain 94; WDR94; activating molecule in beclin-1-regulated autophagy; MGC33725; |
Gene ID : | 55626 |
mRNA Refseq : | NM_017749 |
Protein Refseq : | NP_060219 |
MIM : | 611359 |
UniProt ID : | Q9C0C7 |
Products Types
◆ Recombinant Protein | ||
AMBRA1-332H | Recombinant Human AMBRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
AMBRA1-01H | Active Recombinant Human AMBRA1 Protein, Myc/DDK-tagged | +Inquiry |
Ambra1-1608M | Recombinant Mouse Ambra1 Protein, Myc/DDK-tagged | +Inquiry |
AMBRA1-501M | Recombinant Mouse AMBRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Ambra1-3111M | Recombinant Mouse Ambra1, His-tagged | +Inquiry |
◆ Lysates | ||
AMBRA1-8887HCL | Recombinant Human AMBRA1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMBRA1 undergoes post-translational modifications, including phosphorylation and ubiquitination, which regulate its interaction with other autophagy-related proteins and modulate its function in autophagy.
AMBRA1 participates in selective autophagy processes, such as mitophagy (selective clearance of damaged mitochondria) and aggrephagy (clearance of protein aggregates), contributing to cellular quality control mechanisms.
AMBRA1 plays a critical role in autophagy regulation by promoting autophagosome formation, facilitating cargo recognition and degradation, and coordinating the activity of other autophagy-related proteins.
AMBRA1 facilitates the clearance of protein aggregates and damaged organelles through autophagy, preventing their accumulation and mitigating cellular damage.
AMBRA1 expression is induced during autophagy by various signaling pathways, including nutrient deprivation, cellular stress, and growth factor signaling.
In addition to its role in autophagy, AMBRA1 interacts with other cellular processes, such as apoptosis and cell proliferation, forming intricate crosstalk that influences cellular fate and homeostasis.
AMBRA1 interacts with Beclin 1, ULK1, and ATG proteins through protein-protein interactions, forming complexes that regulate autophagy initiation and autophagosome formation.
Depletion of AMBRA1 leads to impaired autophagy activity, resulting in the accumulation of damaged proteins and organelles and compromised cellular homeostasis.
AMBRA1 promotes autophagosome formation by regulating the nucleation and elongation steps, ensuring proper assembly of the autophagy machinery and cargo sequestration.
The activity of AMBRA1 in autophagy is modulated by upstream signaling pathways, including mTORC1, AMPK, and MAPK pathways, which integrate nutrient availability and cellular stresses.
Customer Reviews (3)
Write a reviewEasy integration into multi-omics workflows for comprehensive analysis.
Wide dynamic range for accurate quantification of protein changes.
Low false-positive rate in target protein identification.
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