Active Recombinant Human CD27 protein, mFc-tagged

• Cat.No.: CD27-2955H
• Product Overview: Recombinant Human CD27 protein(Thr 21-Ile 192), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human CD27 Protein, Mouse IgG2a Fc Tag at 2 μg/mL (100 μL/well) can bind Human CD27 Ligand Protein, Fc Tag with a linear range of 0.2-4 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 46.2 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Thr 21-Ile 192
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD27 CD27 molecule [ Homo sapiens ]
• Official Symbol: CD27
• Synonyms: CD27; CD27 molecule; TNFRSF7, tumor necrosis factor receptor superfamily, member 7; CD27 antigen; S152; Tp55; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; T14; TNFRSF7; MGC20393
• Gene ID: 939
• mRNA Refseq: NM_001242
• Protein Refseq: NP_001233
• MIM: 186711
• UniProt ID: P26842