Active Recombinant Human CDC42BPA(1-473) Protein, C-His-tagged

• Cat.No.: CDC42BPA-157H
• Product Overview: C-terminal 6Histagged, recombinant, human MRCKα, amino acids 1–473, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MRCKα Protein is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-473 aa
• Form: 1.188mg/ml of enzyme in of 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 246U/mg, where one unit of MRCKα, active activity is defined as 1nmol phosphate incorporated into 100μM (KKRNRTLTV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 55.3 kDa
• AA Sequence: 1 MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 61 KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 121 CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 181 YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 241 PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 301 FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 361 EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 421 GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQHHHHHH
• Purity: Purity 91.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDC42BPA CDC42 binding protein kinase alpha [ Homo sapiens (human) ]
• Official Symbol: CDC42BPA
• Synonyms: CDC42BPA; CDC42 binding protein kinase alpha; MRCK; MRCKA; PK428; MRCKalpha
• Gene ID: 8476
• mRNA Refseq: NM_001366010.2
• Protein Refseq: NP_001352939.1
• MIM: 603412
• UniProt ID: Q5VT25