Active Recombinant Human CDK15 (Y103-F387) and CCNB1 (M1-V433) Protein, N-GST/6×His tagged

• Cat.No.: CDK15-01H
• Product Overview: Human CDK15, internal fragment, amino acids Y103-F387 (as in NCBI/Protein entry NP_001248364.1), N-terminal GST-HIS6 fusion protein with a 3C cleavage site and human cyclin B1, full length, amino acids M1-V433 (as in NCBI/Protein entry NP_114172.4), N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, co-expressed in Sf9 insect cells

Specification

• Source: Sf9 insect cells
• Species: Human
• Tag: N-GST/6×His
• Bio-activity: Specific kinase activity (Pi transfer): 1.9 pmol/μg × min
  ATP-KM: 2.8 μM
• Molecular Mass: Theoretical MWCDK15: 60,389 Da
  Theoretical MWCycB1: 78,919 Da
• Protein Length: CDK15: Y103-F387
  CCNB1:M1-V433
• Storage: At -80 centigrade
  For complete recovery, mix well and spin before use. Product must not be stored in diluted solutions, aliquots below 10 μL are not advisable. Avoid repeated freeze-thaw cycles!
• Concentration: 0.156 μg/μL (Bradford method using BSA as standard protein)
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20 % glycerol

Gene Information

• Gene Name: CDK15 cyclin dependent kinase 15 [ Homo sapiens (human) ]
• Official Symbol: CDK15
• Synonyms: CDK15; cyclin dependent kinase 15; PFTK2; ALS2CR7; PFTAIRE2; cyclin-dependent kinase 15; PFTAIRE protein kinase 2; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein; cell division protein kinase 15; serine/threonine protein kinase; serine/threonine-protein kinase ALS2CR7; serine/threonine-protein kinase PFTAIRE-2; EC 2.7.11.22
• Gene ID: 65061
• mRNA Refseq: NM_001261435
• Protein Refseq: NP_001248364
• MIM: 616147
• UniProt ID: Q96Q40