Active Recombinant Human CSF1 Protein (Glu33-Ser190), C-His tagged, Animal-free, Carrier-free

• Cat.No.: CSF1-27H
• Product Overview: Recombinant Human CSF1 Protein (Glu33-Ser190) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Macrophage Colony Stimulating Factor (M-CSF) is a 18.54 kDa member of hematopoietic Growth Factors with 159 amino acid residues. M-CSF produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant human M-CSF is approximately >2.5 × 10⁸ IU/mg.
• Molecular Mass: The protein has a calculated MW of 13.34 kDa.
  The protein migrates as 13-26 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Glu33-Ser190
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens (human) ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603