Active Recombinant Human CSF1R protein, His-tagged

• Cat.No.: CSF1R-1268H
• Product Overview: Active Recombinant Human CSF1R/M-CSF R/CD115 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile20-Glu512) of human CSF1R (Accession #NP_005202.2) fused with a 6×His tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human M-CSF at 2 μg/mL (100 μL/well) can bind Recombinant Human CSF1R with a linear range of 50-200 ng/mL.
  2.Measured by its ability to inhibit the GCSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this ettect is typically 0.095-0.38 ng/mL.
  3.Measured by its binding ability in a functional ELISA.Immobilized Human CSF-1/M-CSF at 2μg/mL (100 μL/well) can bind Human CSF1R with a linear range of 2-185 ng/mL.
• Molecular Mass: 70-100 kDa
• Protein Length: Ile20-Glu512
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: CSF1R colony stimulating factor 1 receptor [ Homo sapiens ]
• Official Symbol: CSF1R
• Synonyms: CSF1R; colony stimulating factor 1 receptor; FMS, McDonough feline sarcoma viral (v fms) oncogene homolog; macrophage colony-stimulating factor 1 receptor; C FMS; CD115; CSFR; CSF-1-R; CD115 antigen; CSF-1 receptor; FMS proto-oncogene; proto-oncogene c-Fms; macrophage colony stimulating factor I receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; FMS; FIM2; HDLS; C-FMS; CSF-1R; M-CSF-R
• Gene ID: 1436
• mRNA Refseq: NM_005211
• Protein Refseq: NP_005202
• MIM: 164770
• UniProt ID: P07333