| Description : |
DDR1 is a tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
492-end |
| Form : |
0.264mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 20mM βglycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : |
396U/mg, where one unit of DDR1, activated activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
50 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF NPPHSAPCVP NGSALLLSNP AYRLLLATYA 61 RPPRGPGPPT PAWAKPTNTQ AYSGDYMEPE KPGAPLLPPP PQNSVPHYAE ADIVTLQGVT 121 GGNTYAVPAL PPGAVGDGPP RVDFPRSRLR FKEKLGEGQF GEVHLCEVDS PQDLVSLDFP 181 LNVRKGHPLL VAVKILRPDA TKNARNDFLK EVKIMSRLKD PNIIRLLGVC VQDDPLCMIT 241 DYMENGDLNQ FLSAHQLEDK AAEGAPGDGQ AAQGPTISYP MLLHVAAQIA SGMRYLATLN 301 FVHRDLATRN CLVGENFTIK IADFGMSRNL YAGDYYRVQG RAVLPIRWMA WECILMGKFT 361 TASDVWAFGV TLWEVLMLCR AQPFGQLTDE QVIENAGEFF RDQGRQVYLS RPPACPQGLY 421 ELMLRCWSRE SEQRPPFSQL HRFLAEDALN TV |
| Purity : |
Purity 77% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
