Active Recombinant Human DHODH protein, His-tagged

• Cat.No.: DHODH-11971H
• Product Overview: Recombinant Human DHODH protein(NP_001352.2)(Arg35~Asp392) was expressed in E.coli with a N-terminal His tag.

Specification

• Description: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: The binding activity of DHODH with FKBP8.
• Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg35~Asp392
• AA Sequence: RFYAEHLMPTLQGLLDPESAHRLAV RFTSLGLLPRARFQDSDMLEVRVLG HKFRNPVGIAAGFDKHGEAVDGLYK MGFGFVEIGSVTPKPQEGNPRPRVF RLPEDQAVINRYGFNSHGLSVVEHR LRARQQKQAKLTEDGLPLGVNLGKN KTSVDAAEDYAEGVRVLGPLADYLV VNVSSPNTAGLRSLQGKAELRRLLT KVLQERDGLRRVHRPAVLVKIAPDL TSQDKEDIASVVKELGIDGLIVTNT TVSRPAGLQGALRSETGGLSGKPLR DLSTQTIREMYALTQGRVPIIGVGG VSSGQDALEKIRAGASLVQLYTALT FWGPPVVGKVKRELEALLKEQGFGG VTDAIGAD
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  (May be suitable for use in other assays to be determined by the end user.)
• Notes: The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8°C for one month.
  Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DHODH dihydroorotate dehydrogenase (quinone)[Homo sapiens (human)]
• Official Symbol: DHODH
• Synonyms: DHOdehase; Dihydroorotate oxidase; Dihydroorotate dehydrogenase (quinone), mitochondrial
• Gene ID: 1723
• mRNA Refseq: NM_001361.5
• Protein Refseq: NP_001352.2
• MIM: 126064
• UniProt ID: Q02127

Reviews

07/29/2020

Saves time and effort. Highly recommended for research.

11/03/2017

Top-notch support for insights. Trusted for accurate data.

05/05/2017

Reliable data, essential for our protein research.

Q&As

How does DHODH contribute to pyrimidine biosynthesis?

It catalyzes a key step in the pyrimidine biosynthesis pathway, converting dihydroorotate to orotate.

What is the primary role of DHODH in cellular metabolism?

DHODH, dihydroorotate dehydrogenase, is crucial for the de novo synthesis of pyrimidines, essential for DNA and RNA synthesis.

How does DHODH interact with other enzymes in the pyrimidine biosynthesis pathway?

DHODH collaborates with other enzymes in pyrimidine biosynthesis, playing a role in nucleotide metabolism.

What role does DHODH play in cell proliferation and growth?

DHODH is important for cell proliferation, as pyrimidine synthesis is essential for DNA replication and cell growth.

How do genetic variations in DHODH affect its function in nucleotide synthesis?

Genetic mutations in DHODH can disrupt nucleotide synthesis, affecting cellular replication and function.

What is the impact of altered DHODH activity on autoimmune diseases?

Altered DHODH activity is linked with autoimmune conditions, as it affects lymphocyte proliferation and function.

What potential therapeutic applications arise from targeting DHODH in disease treatment?

Targeting DHODH in therapies could be effective in treating autoimmune diseases and certain cancers by modulating pyrimidine synthesis.