Active Recombinant Human EGF Protein (Asn971-Arg1023), GMP Grade, Animal-Free

• Cat.No.: EGF-34HG
• Product Overview: GMP recombinant human EGF protein (Asn971-Arg1023) with an N-terminal Met was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Description: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-100 pg/mL.
  The specific activity of Recombinant Human EGF is >8.0 x 105 IU/mg, which is calibrated against the human EGF WHO International Standard.
• Molecular Mass: Predicted Molecular Mass: 6 kDa
  SDS-PAGE: 6 kDa, reducing conditions
• Protein Length: Asn971-Arg1023
• N-terminal Sequence Analysis: Met-Asn971-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4
• Gene ID: 1950
• mRNA Refseq: NM_001178130
• Protein Refseq: NP_001171601
• MIM: 131530
• UniProt ID: P01133