Active Recombinant Human EPHA3(578-end) Protein, N-His-tagged

• Cat.No.: EPHA3-67H
• Product Overview: N-terminal 6His tagged, recombinant, human EphA3 amino acids 578–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ - NTA agarose.

Specification

• Description: Eph family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Recruitment of CrkII and activation of Rho Signaling are responsible for EphA3-mediated cell rounding, blebbing and de-adhesion. This response is activated by ephrin-A5-mediated receptor clustering and EphA3 tyrosine kinase activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 578-end
• Form: 1.19mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 10mM benzamidine, 1mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 91U/mg, where one unit of EphA3, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 49 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMSLGS EKRLHFGNGH LKLPGLRTYV DPHTYEDPTQ 61 AVHEFAKELD ATNISIDKVV GAGEFGEVCS GRLKLPSKKE ISVAIKTLKV GYTEKQRRDF 121 LGEASIMGQF DHPNIIRLEG VVTKSKPVMI VTEYMENGSL DSFLRKHDAQ FTVIQLVGML 181 RGIASGMKYL SDMGYVHRDL AARNILINSN LVCKVSDFGL SRVLEDDPEA AYTTRGGKIP 241 IRWTSPEAIA YRKFTSASDV WSYGIVLWEV MSYGERPYWE MSNQDVIKAV DEGYRLPPPM 301 DCPAALYQLM LDCWQKDRNN RPKFEQIVSI LDKLIRNPGS LKIITSAAAR PSNLLLDQSN 361 VDITTFRTTG DWLNGVWTAH CKEIFTGVEY SSCDTIAKIS TDDMKKVGVT VVGPQKKIIS 421 SIKALETQSK NGPVPV
• Purity: Purity 77% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: EPHA3 EPH receptor A3 [ Homo sapiens (human) ]
• Official Symbol: EPHA3
• Synonyms: EPHA3; EPH receptor A3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
• Gene ID: 2042
• mRNA Refseq: NM_005233.6
• Protein Refseq: NP_005224.2
• MIM: 179611
• UniProt ID: P29320