Active Recombinant Human EPHA7(613-909) Protein, N-His-tagged

• Cat.No.: EPHA7-70H
• Product Overview: N-terminal 6Histagged, recombinant human EphA7, amino acids 613–909, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: EphA7 (EPH Receptor A7) belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 613-909 aa
• Form: 2.774mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 53U/mg, where one unit of EphA7, active activity is defined as 1nmol phosphate incorporated into 100μM PDKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 38 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQTYED PNRAVHQFAK ELDASCIKIE 61 RVIGAGEFGE VCSGRLKLPG KRDVAVAIKT LKVGYTEKQR RDFLCEASIM GQFDHPNVVH 121 LEGVVTRGKP VMIVIEFMEN GALDAFLRKH DGQFTVIQLV GMLRGIAAGM RYLADMGYVH 181 RDLAARNILV NSNLVCKVSD FGLSRVIEDD PEAVYTTTGG KIPVRWTAPE AIQYRKFTSA 241 SDVWSYGIVM WEVMSYGERP YWDMSNQDVI KAIEEGYRLP APMDCPAGLH QLMLDCWQKE 301 RAERPKFEQI VGILDKMIRN PNSLKTPLGT CSR
• Purity: Purity 98% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: EPHA7 EPH receptor A7 [ Homo sapiens (human) ]
• Official Symbol: EPHA7
• Synonyms: EPHA7; EPH receptor A7; EHK3; EK11; EHK-3; HEK11
• Gene ID: 2045
• mRNA Refseq: NM_001288629.2
• Protein Refseq: NP_001275558.1
• MIM: 602190
• UniProt ID: Q15375