Active Recombinant Human GPC3 Protein, His-tagged, PE-Labeled

• Cat.No.: GPC3-4662H
• Product Overview: PE-Labeled Recombinant Human Protein(Gln 25 - His 559), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: 5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Glypican 3, His Tag and negative control protein respectively. PE signal was used to evaluate the binding activity.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 64.5 kDa.
• Protein Length: Gln 25 - His 559
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; SDYS; glypican-3; DGSX; glypican proteoglycan 3; OCI 5; SGB; SGBS; SGBS1; secreted glypican-3; intestinal protein OCI-5; heparan sulphate proteoglycan; MXR7; OCI-5; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_001164617
• Protein Refseq: NP_001158089
• MIM: 300037
• UniProt ID: P51654