| Description : |
Human Interferon gamma (hIFN-γ) is amacrophage‐activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ–induced inflammatory cascade summons a variety of immune‐related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50< 0.05 ng/mL, measured by cytotoxicity assay using HT‐29 cells. |
| Molecular Mass : |
17kDa, observed by reducing SDS‐PAGE. |
| Protein Length : |
144 |
| AA Sequence : |
MQDPYVKEAENLKKYFNAGHSDVAD NGTLFLGILKNWKEESDRKIMQSQI VSFYFKLFKNFKDDQSIQKSVETIK EDMNVKFFNSNKKKRDDFEKLTNYS VTDLNVQRKAIHELIQVMAELSPAA KTGKRKRSQMLFRGRRASQ |
| Endotoxin : |
< 1 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by reducing SDS‐PAGE. |
| Storage : |
Lyophilized recombinant human Interferon gamma (rhIFN‐γ) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhIFN-γ should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
