| Description : |
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the type II class of interferons. IFNγ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. IFNγ is critical for innate and adaptive immunity against viral, protozoal, bacterial infections. IFNγ is secreted by Th1, cytotoxic T cells, NKT cells and NK cells. IFNγ is particularly suitable for human CIK activation along with IL-2. |
| Source : |
Human cells |
| Species : |
Human |
| Bio-activity : |
IFNγ stimulates STAT1 phosphorylation in human lung microvascular endothelial cells. HLMVECs were serum starved in EBM-2 medium containing 2% FBS for overnight then treated with La IFNγ for 5 minutes at the indicated concentrations ranging from 0.5 to 500 ng/mL. Phosphorylated STAT1 (Tyr701) was detected by phosphor STAT1 antibody by Western Blot. |
| Molecular Mass : |
21-25 kDa |
| Endotoxin : |
<0.5 EU/ μg |
| Purity : |
High Purity, >95% by SDS-PAGE |
| Stability : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein |
| Reconstitution : |
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
