Source : |
E. coli |
Species : |
Human |
Tag : |
N/A |
Protein length : |
Asn49-Ser162 |
Form : |
Lyophilized from sterile 50 mM Tris, 200 mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
1. Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is typically 2-8 ng/mL. 2. Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated bound to Anti-IL15 Antibody was 0.1nM. |
Molecular Mass : |
The recombinant human IL15 consists of 114 amino acids and predicts a molecular mass of 12.8 kDa. |
Endotoxin : |
< 1 EU per μg of the protein as determined by the LAL method. |
Purity : |
> 95 % as determined by SDS-PAGE. |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |