Active Recombinant Human IL15 protein, Fc-Avi-tagged, Biotinylated
Cat.No. : | IL15-272H |
Product Overview : | Biotinylated Recombinant Human IL15 protein(Asn 49 - Ser 162), fused with Fc and Avi tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc-Avi |
Form : | Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human IL-15 R alpha, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15, Fc,Avitag with a linear range of 0.5-8 ng/mL. |
Molecular Mass : | This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 41.0 kDa. The protein migrates as 54-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : | Asn49-Ser162 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | IL15 interleukin 15 [ Homo sapiens ] |
Official Symbol : | IL15 |
Synonyms : | IL15; interleukin 15; interleukin-15; IL 15; MGC9721; IL-15; |
Gene ID : | 3600 |
mRNA Refseq : | NM_000585 |
Protein Refseq : | NP_000576 |
MIM : | 600554 |
UniProt ID : | P40933 |
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◆ Lysates | ||
IL15-5248HCL | Recombinant Human IL15 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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