| Description : |
Interleukin 17F is a disulfide-linked homodimeric protein belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL-17 family is inducible by IL15 and IL23, and IL17F induces T-cell and PBMC activation and proliferation. It also performs cartilage matrix turnover and angiogenesis prevention. Out of the IL-17 family, IL17F is the most homologous to IL17 and dimerizes in a way similar to that of nerve growth factors (NGF) and other neurotrophins. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein) |
| Bio-activity : |
The ED50 is 8.0-40.0ng/mL as determined by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. |
| Molecular Mass : |
15.2 kDa |
| Endotoxin : |
<1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Purity : |
>95% |
| Applications : |
Cell Assay |
| Storage : |
The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade - 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
Reconstitute in sterile 4mM HCl at 25μg/mL (containing at least 0.1% human or bovine serum albumin) |
