Active Recombinant Human IL1B Protein (153 aa)
Cat.No. : | IL1B-101I |
Product Overview : | Recombinant Human IL1B Protein without tag was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | IL-1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. |
Source : | E. coli |
Species : | Human |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine D10S cells is less than 1.0 pg/mL, corresponding to a Specific Activity of >1.0 × 10^9 IU/mg. |
Molecular Mass : | Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
Protein Length : | 153 |
AA Sequence : | APVRSLNCTLRDSQQKSLVMSGPYE LKALHLQGQDMEQQVVFSMSFVQGE ESNDKIPVALGLKEKNLYLSCVLKD DKPTLQLESVDPKNYPKKKMEKRFV FNKIEINNKLEFESAQFPNWYISTS QAENMPVFLGGTKGGQDITDFTMQF VSS |
Endotoxin : | Less than 1 EU/μg of rHuIL-1β as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analyses. |
Storage : | This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.5. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | IL1B interleukin 1, beta [ Homo sapiens ] |
Official Symbol : | IL1B |
Synonyms : | IL1B; interleukin 1, beta; interleukin-1 beta; IL 1B; IL1 BETA; IL1F2; IL-1 beta; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; IL-1; IL1-BETA; |
Gene ID : | 3553 |
mRNA Refseq : | NM_000576 |
Protein Refseq : | NP_000567 |
MIM : | 147720 |
UniProt ID : | P01584 |
Products Types
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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