| Description : |
Interleukin 1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. |
| Source : |
CHO |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50< 10 pg/mL, measured in a cell proliferation assay using D10S cells, corresponding to a specific activity of >1 × 10^8 units/mg. |
| Molecular Mass : |
17kDa, observed by non-reducing SDS-PAGE. |
| AA Sequence : |
APVRSLNCTLRDSQQKSLVMSGPYE LKALHLQGQDMEQQVVFSMSFVQGE ESNDKIPVALGLKEKNLYLSCVLKD DKPTLQLESVDPKNYPKKKMEKRFV FNKIEINNKLEFESAQFPNWYISTS QAENMPVFLGGTKGGQDITDFTMQF VSS |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : |
Lyophilized recombinant human Interleukin 1 beta (IL-1 beta) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
