Active Recombinant Human IL21R protein, hFc-tagged

• Cat.No.: IL21R-245H
• Product Overview: Recombinant Human IL21R protein(NP_068570.1)(Met1-Pro236), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its ability to inhibit IL21-induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 40-200 ng/mL in the presence of 2 ng/mL of recombinant human IL21.2. Immobilized Recombinant Human Interleukin-21 / IL-21 Protein(Cat:GMP-10584-HNAE) at 2μg/mL (100μL/well) can bind Recombinant Human IL-21R / Il21R Protein (Fc Tag)(Cat:11483-H02H), the EC50 is 130-480 ng/mL.
• Molecular Mass: The recombinant human IL21R consists of 458 amino acids and predicts a molecular mass of 52 kDa.
• Protein Length: Met1-Pro236
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL21R interleukin 21 receptor [ Homo sapiens ]
• Official Symbol: IL21R
• Synonyms: IL21R; interleukin 21 receptor; interleukin-21 receptor; CD360; IL-21R; IL-21 receptor; novel interleukin receptor; NILR; MGC10967
• Gene ID: 50615
• mRNA Refseq: NM_021798
• Protein Refseq: NP_068570
• MIM: 605383
• UniProt ID: Q9HBE5