Active Recombinant Human INSRR(944-1266) Protein, N-His-tagged

• Cat.No.: INSRR-113H
• Product Overview: N-terminal 6Histagged, recombinant, human IRR, amino acids 944–1266 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ - NTA agarose.

Specification

• Description: IRR protein is a receptor with tyrosine-protein kinase activity. It functions as a pH sensing receptor which is activated by increased extracellular pH. It activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 944-1266 aa
• Form: 0.566mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 42U/mg, where one unit of IRR, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 37.9 kDa
• AA Sequence: 1 MHHHHHHEFY GKKRNRTLYA SVNPEYFSAS DMYVPDEWEV PREQISIIRE LGQGSFGMVY 61 EGLARGLEAG EESTPVALKT VNELASPREC IEFLKEASVM KAFKCHHVVR LLGVVSQGQP 121 TLVIMELMTR GDLKSHLRSL RPEAENNPGL PQPALGEMIQ MAGEIADGMA YLAANKFVHR 181 DLAARNCMVS QDFTVKIGDF GMTRDVYETD YYRKGGKGLL PVRWMAPESL KDGIFTTHSD 241 VWSFGVVLWE IVTLAEQPYQ GLSNEQVLKF VMDGGVLEEL EGCPLQLQEL MSRCWQPNPR 301 LRPSFTHILD SIQEELRPSF RLLSFYYSPE CR
• Purity: Purity 71.2% by SDS PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: INSRR insulin receptor related receptor [ Homo sapiens (human) ]
• Official Symbol: INSRR
• Synonyms: INSRR; insulin receptor related receptor; IRR
• Gene ID: 3645
• mRNA Refseq: NM_014215.3
• Protein Refseq: NP_055030.1
• MIM: 147671
• UniProt ID: P14616