Active Recombinant Human MET(Y1248D)(974-end) Mutation Protein, N-His-tagged
Cat.No. : | MET-141H |
Product Overview : | N-terminal 6His-tagged, recombinant, human, Met amino acids 974–end, containing the mutation Y1248D, expressed by baculovirus in Sf21 insect cells. Purified using Ni 2+/NTA agarose. Met Y1248D is a germline mutation that has been identified in patients with hereditary papillary renal carcinomas (HPRC). In vitro studies have demonstrated that this mutation has a moderate transforming ability. |
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- Gene Information
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Description : | N-terminal 6His-tagged recombinant, human Met amino acids 974-end, containing the mutation Y1248D. Met Y1248D is a germline mutation that has been identified in patients with hereditary papillary renal carcinomas (HPRC). in vitro studies have demonstrated that this mutation has a moderate transforming ability. |
Source : | Sf21 |
Species : | Human |
Tag : | His |
Protein length : | 974-end |
Form : | 1.899mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : | 1961U/mg, where one unit of Met (Y1248D) activity is defined as 1nmol phosphate incorporated into 250μM (KKKGQEEEYVFIE) per minute at 30 centigrade with a final ATP concentration of 100μM. |
Molecular Mass : | 50 kDa |
AA Sequence : | 1 MHHHHHHKKR KQIKDLGSEL VRYDARVHTP HLDRLVSARS VSPTTEMVSN ESVDYRATFP 61 EDQFPNSSQN GSCRQVQYPL TDMSPILTSG DSDISSPLLQ NTVHIDLSAL NPELVQAVQH 121 VVIGPSSLIV HFNEVIGRGH FGCVYHGTLL DNDGKKIHCA VKSLNRITDI GEVSQFLTEG 181 IIMKDFSHPN VLSLLGICLR SEGSPLVVLP YMKHGDLRNF IRNETHNPTV KDLIGFGLQV 241 AKGMKYLASK KFVHRDLAAR NCMLDEKFTV KVADFGLARD MDDKEYYSVH NKTGAKLPVK 301 WMALESLQTQ KFTTKSDVWS FGVLLWELMT RGAPPYPDVN TFDITVYLLQ GRRLLQPEYC 361 PDPLYEVMLK CWHPKAEMRP SFSELVSRIS AIFSTFIGEH YVHVNATYVN VKCVAPYPSL 421 LSSEDNADDE VDTRPASFWE TS |
Purity : | Purity 74% by SDS-PAGE and Coomassie blue staining. |
Applications : | Drug Discovery & Development |
Storage : | 6 months at -70 centigrade. |
Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
Gene Name : | MET MET proto-oncogene, receptor tyrosine kinase [ Homo sapiens (human) ] |
Official Symbol : | MET |
Synonyms : | MET; MET proto-oncogene, receptor tyrosine kinase; DA11; HGFR; AUTS9; RCCP2; c-Met; DFNB97 |
Gene ID : | 4233 |
mRNA Refseq : | NM_000245.4 |
Protein Refseq : | NP_000236.2 |
MIM : | 164860 |
UniProt ID : | P08581 |
Products Types
◆ Recombinant Protein | ||
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MET-1139M | Recombinant Mouse MET protein(Met1-Asn929), His-tagged | +Inquiry |
MET-947H | Recombinant Human MET Protein, MYC/DDK-tagged | +Inquiry |
MET-219H | Recombinant Human MET Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
MET-001HCL | Recombinant Human MET cell lysate | +Inquiry |
MET-2047MCL | Recombinant Mouse MET cell lysate | +Inquiry |
MET-1054RCL | Recombinant Rat MET cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-0550 | Met Kinase (Human) Assay/Inhibitor Screening Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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