Active Recombinant Human N-Acylsphingosine Amidohydrolase (non-lysosomal ceramidase) 2, His-tagged

• Cat.No.: ASAH2-582H
• Product Overview: Recombinant Human ASAH2protein was expressed in Chinese Hamster Ovary cell line with a N-terminal 6-Histag. Thr15-Ile761.

Specification

• Cat. No.: ASAH2-582H
• Description: The human ASAH2 geneencodes Nacylsphingosine amidohydrolase2, also known as neutral ornonlysosomal ceramidase. ASAH2 is a type II integral membrane protein thatcan be cleaved to produce a soluble secreted protein. The enzyme is abundantin the brush border membranes of the intestine, but is also expressed intissues such as kidney, brain and liver. An Nterminally truncated form ofhuman ASAH2 has been shown to localize to mitochondria.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Supplied as a 0.2 μmfiltered solution in MES, NaCl and Sodium Cholate.
• N-terminalSequence: His
• Molecular Weight: 83 kDa
• Activity: Measured by itsability to hydrolyze the substrate C12:0 ceramide into sphingosine anddodecanoic acid. The specific activity is > 5,000 pmoles/min/μg, asmeasured under the described conditions.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 132 kDa,reducing conditions.
• Purity: > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter opening.

Gene Information

• Gene Name: ASAH2 N-acylsphingosineamidohydrolase (non-lysosomal ceramidase) 2 [ Homo sapiens ]
• Official Symbol: ASAH2
• Synonyms: ASAH2;N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; HNAC1; BCDase;LCDase; NCDase; N-Cdase; neutral ceramidase; mitochondrial ceramidase;non-lysosomal ceramidase; acylsphingosine deacylase 2; neutral/alkalineceramidase; N-acylsphingosine amidohydrolase 2; EC 3.5.1.23
• Gene ID: 56624
• mRNA Refseq: NM_001143974
• Protein Refseq: NP_001137446
• MIM: 611202
• UniProt ID: Q9NR71
• Chromosome Location: 10q11.23
• Pathway: Glycosphingolipidmetabolism; Metabolic pathways; Metabolism; Metabolism of lipids andlipoproteins; Sphingolipid metabolism; Sphingosine biosynthesis; sphingolipidmetabolism
• Function: ceramidase activity;hydrolase activity

Reviews

04/04/2019

It reliably and accurately detects and quantifies target molecules, allowing for precise measurement of analytes in my samples.

02/25/2019

I highly recommend the ASAH2 Protein for its outstanding performance in ELISA assays.

11/22/2016

The sensitivity and specificity of the ASAH2 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

Q&As

Can ASAH2 activity be regulated by post-translational modifications?

Post-translational modifications, such as phosphorylation, may impact ASAH2 activity, although further research is needed for a comprehensive understanding.

Are there any known inhibitors of ASAH2?

While specific inhibitors for ASAH2 are not yet available, researchers are actively exploring potential compounds that can modulate its activity.

In which cellular compartments is ASAH2 found?

ASAH2 is primarily localized in the lysosomes, where it carries out the ceramide hydrolysis process.

What are the consequences of ASAH2 deficiency?

Deficiency in ASAH2 activity can lead to the accumulation of ceramides, which may disrupt cellular signaling and contribute to disease development.

How is ASAH2 gene expression regulated?

ASAH2 gene expression can be regulated by various factors, including transcription factors, epigenetic modifications, and signaling pathways.

Are there any diseases associated with ASAH2?

Mutations in the ASAH2 gene have been associated with a rare form of hereditary sensory and autonomic neuropathy (HSAN) called HSAN1C.

Is ASAH2 ubiquitously expressed in all tissues?

Yes, ASAH2 is ubiquitously expressed in various tissues throughout the body.