| Description : |
Three tyrosine kinase receptors, designated TRKA, TRKB and TRKC, have been identified that selectively bind NGF, BDNF and NT-3, respectively; NT-4/5 also binds to the TRKB (TrkB tyrosine kinase, Trk-B, GP145-TrkB, BDNF/NT growth factors12) receptor. NGF binds to their specific TRK receptor to stimulate intracellular signaling pathways leading to specific protein phosphorylations and differential gene inductions. TRK receptor kinases engage the activation of a branched proto-oncoprotein signaling pathway involving Shc, Src, Ras, Raf, mitogen-activated protein kinase, and other downstream effectors. Inhibitors of TRKB may be useful in the treatment of cancer as well as anorexia and bulimia nervosa. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
455-end |
| Form : |
0.76mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
371U/mg, where one unit of TrkB, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM and an enzyme concentration of 171 ng/well. |
| Molecular Mass : |
42.9 kDa |
| AA Sequence : |
1 MHHHHHHEFK LARHSKFGMK GPASVISNDD DSASPLHHIS NGSNTPSSSE GGPDAVIIGM 61 TKIPVIENPQ YFGITNSQLK PDTFVQHIKR HNIVLKRELG EGAFGKVFLA ECYNLCPEQD 121 KILVAVKTLK DASDNARKDF HREAELLTNL QHEHIVKFYG VCVEGDPLIM VFEYMKHGDL 181 NKFLRAHGPD AVLMAEGNPP TELTQSQMLH IAQQIAAGMV YLASQHFVHR DLATRNCLVG 241 ENLLVKIGDF GMSRDVYSTD YYRVGGHTML PIRWMPPESI MYRKFTTESD VWSLGVVLWE 301 IFTYGKQPWY QLSNNEVIEC ITQGRVLQRP RTCPQEVYEL MLGCWQREPH MRKNIKGIHT 361 LLQNLAKASP VYLDILG |
| Purity : |
Purity 90% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
