Active Recombinant Human RSPO1 protein
Cat.No. : | RSPO1-8392H |
Product Overview : | Recombinant Human RSPO1 was expressed in CHO. |
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Description : | R-Spondin-1 (Rspo-1) belongs to the (Rspo) family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing the furin-like and thrombospondin structural domains. Rspo-1 is expressed in certain areas of the developing central nervous system, as well as in the adrenal glands, ovary, testis, thyroid, and trachea. Rspo can interact with the Frizzled/LRP6 receptor complex in a manner that stimulates the Wnt/beta-catenin signaling pathway. |
Source : | CHO |
Species : | Human |
Bio-activity : | R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The expected ED50 is 1.0-3.0 ug/ml. |
Molecular Mass : | R-Spondin-1 is a 26.7 kDa protein consisting of 243 amino acid residues. Due to glycosylation, R-Spondin-1 migrates at an apparent molecular weight of approximately 40.0 kDa by SDS-PAGE analysis under reducing conditions. |
Protein length : | 243 amino acid |
AA Sequence : | SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA |
Purity : | Greater than 95% by SDS-PAGE gel and HPLC analyses. |
Gene Name : | RSPO1 R-spondin 1 [ Homo sapiens ] |
Official Symbol : | RSPO1 |
Synonyms : | RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1; |
Gene ID : | 284654 |
mRNA Refseq : | NM_001038633 |
Protein Refseq : | NP_001033722 |
MIM : | 609595 |
UniProt ID : | Q2MKA7 |
Chromosome Location : | 1p34.2 |
Pathway : | Wnt signaling network, organism-specific biosystem; |
Function : | heparin binding; protein binding; receptor binding; |
Products Types
◆ Recombinant Protein | ||
RSPO1-132H | Active Recombinant Human RSPO1 Protein (Ser21-Ala263), N-His-SUMO tagged, Animal-free, Carrier-free | +Inquiry |
RSPO1-421H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
RSPO1-34H | Recombinant Human RSPO1 Protein | +Inquiry |
RSPO1-28H | Active Recombinant Human RSPO1 Protein, Pre-aliquoted | +Inquiry |
Rspo1-8672M | Active Recombinant Mouse Rspo1 protein(Met1-Gln265), His-tagged | +Inquiry |
◆ Lysates | ||
RSPO1-001MCL | Recombinant Mouse RSPO1 cell lysate | +Inquiry |
RSPO1-001HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-1918HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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