Active Recombinant Human RSPO1 protein, Fc-tagged
Cat.No. : | RSPO1-0660H |
Product Overview : | Recombinant Human RSPO1 protein(Ser21-Ala146), fused with Fc tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized from 0.22 μm filtered solution in 52 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant. |
Bio-activity : | Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag with a linear range of 0.008-0.5 μg/mL. Immobilized Human R-Spondin 1 (21-146), Fc Tag at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag with a linear range of 0.02-0.313 μg/mL. |
Molecular Mass : | This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 40.2 kDa. The protein migrates as 46-56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : | Ser21-Ala146 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | RSPO1 R-spondin 1 [ Homo sapiens ] |
Official Symbol : | RSPO1 |
Synonyms : | RSPO1; R-spondin 1; R spondin homolog (Xenopus laevis); R-spondin-1; FLJ40906; RSPONDIN; R-spondin homolog; roof plate-specific spondin-1; RSPO; CRISTIN3; RP11-566C13.1; |
Gene ID : | 284654 |
mRNA Refseq : | NM_001038633 |
Protein Refseq : | NP_001033722 |
MIM : | 609595 |
UniProt ID : | Q2MKA7 |
Products Types
◆ Lysates | ||
RSPO1-001HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-1918HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-001MCL | Recombinant Mouse RSPO1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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