Active Recombinant Human RXRB, His-tagged

• Cat.No.: RXRB-8492H
• Product Overview: Recombinant Human RXRB, fused with His tag, was expressed in Sf21 Cells.

Specification

• Description: Retinoid X receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain. RXR partners include thyroid hormone receptors (TRs), retinoic acid receptors (RARs), peroxisome proliferator-activated receptor, several constitutive active orphan nuclear receptors, oxysterol receptors, and constitutive androstane receptors. RXRs also form homodimers to mediate the effects of 9-cis-retinoic acid. Depending on these protein-protein interactions, RXR-containing complexes have distinct ligand-dependent and constitutive functions. RXRbeta was originally identified as a protein able to bind to the regulatory region II of the murine major histocompatibility complex (MHC) class I promoter and referred to as H2RIIBP. The human RXRbeta gene has been mapped to the short arm or centromeric region of chromosome 6 (6pterˉq13) which also contains the MHC I locus. Two major RXRbeta isoforms in the mouse, designated RXRbeta1 and RXRbeta2 (GenBank accession numbers D21830 and D21831), differ in the N-terminal (A) domain with RXRbeta1 containing an extra 72 amino acids. These isoform mRNAs are transcribed from two CpG island promoters and the different N-terminal exons are spliced to exons common to both isoforms from the remainder of the gene. Although the two mouse RXR isoforms have been characterised in some detail, less information is available about their human counterparts . Since the expression of different RXR isoforms appears to be well conserved between species, each receptor isoform may hold a specific role in retinoid signalling. However, many RXR expression studies have not recognised the importance of establishing which RXR isoforms are expressed in a particular system. RXRbeta is predominantly involved in retinoid responses in the N-type SH SY 5Y and the S-type SH S EP neuroblastoma cells, both derivatives of a mixed phenotype neuroblastoma cell line.
• Source: Sf21 Cells
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng purified protein. 20 units (ng) are sufficient for an in vitro transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MSWAARPPFL PQRHAAGQCG PVGVRKEMHC GVASRWRRRR PWLDPAAAAA AAVAGGEQQT PEPEPGEAGR DGMGDSGRDS RSPDSSSPNP LPQGVPPPSP PGPPLPPSTA PSLGGSGAPP PPPMPPPPLG SPFPVISSSM GSPGLPPPAP PGFSGPVSSP QINSTVSLPG GGSGPPEDVK PPVLGVRGLH CPPPPGGPGA GKRLCAICGD RSSGKHYGVY SCEGCKGFFK RTIRKDLTYS CRDNKDCTVD KRQRNRCQYC RYQKCLATGM KREAVQEERQ RGKDKDGDGE GAGGAPEEMP VDRILEAELA VEQKSDQGVE GPGGTGGSGS SPNDPVTNIC QAADKQLFTL VEWAKRIPHF SSLPLDDQVI LLRAGWNELL IASFSHRSID VRDGILLATG LHVHRNSAHS AGVGAIFDRV LTELVSKMRD MRMDKTELGC LRAIILFNPD AKGLSNPSEV EVLREKVYAS LETYCKQKYP EQQGRFAKLL LRLPALRSIG LKCLEHLFFF KLIGDTPIDT FLMEMLEAPH QLA
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 450 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: RXRB retinoid X receptor, beta [ Homo sapiens ]
• Official Symbol: RXRB
• Synonyms: RXRB; retinoid X receptor, beta; retinoic acid receptor RXR-beta; H 2RIIBP; NR2B2; nuclear receptor subfamily 2 group B member 2; RCoR 1; retinoid X nuclear receptor beta; MHC class I promoter binding protein; DAUDI6; RCoR-1; H-2RIIBP; MGC1831
• Gene ID: 6257
• mRNA Refseq: NM_021976
• Protein Refseq: NP_068811
• MIM: 180246
• UniProt ID: P28702
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Non-small cell lung cancer, organism-specific biosystem; Non-small cell lung cancer, conserved biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem
• Function: 9-cis retinoic acid receptor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; protein binding; protein heterodimerization activity; receptor activity; retinoic acid receptor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; steroid hormone receptor activity; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription regulatory region DNA binding; vitamin D receptor binding; zinc ion binding

Reviews

10/19/2022

Timely support for protein expression optimization, appreciated assistance.

04/08/2021

High-quality protein characterization, fundamental for our research.

05/25/2018

Accurate protein structural analysis, indispensable for our investigations.

Q&As

How do environmental factors like diet and sunlight exposure affect the activity and expression of RXRB?

Environmental factors like diet and sunlight alter RXRB activity, impacting its role in skin health and metabolic processes.

What are the effects of RXRB dysregulation on cancer development and progression?

Dysregulation of RXRB can contribute to the development and progression of various cancers.

How does RXRB influence the immune response, especially in autoimmune and inflammatory diseases?

RXRB modulates the immune system, affecting autoimmune and inflammatory responses.

How does RXRB contribute to metabolic regulation, particularly in lipid metabolism and obesity?

RXRB is crucial in lipid metabolism, influencing processes related to obesity and metabolic health.

What role does RXRB play in skin health and the development of skin disorders?

RXRB plays a key role in maintaining skin health and is involved in the pathology of skin disorders.

What is the impact of RXRB on neurodevelopment and neurodegenerative diseases?

RXRB impacts neurodevelopment and is implicated in the progression of neurodegenerative diseases.

How does RXRB interact with retinoic acid and other ligands to regulate gene expression?

RXRB binds retinoic acid and other ligands to regulate genes involved in development, metabolism, and cell differentiation.