Active Recombinant Human SEMA6B, Fc Chimera

• Cat.No.: SEMA6B-1925H
• Product Overview: Recombinant Human SEMA6B fussed with a Fc region of human IgG1 at the C-terminus via a peptide linker was expressed in a mouse myeloma cell line,NS0.

Specification

• Cat. No.: SEMA6B-1925H
• Description: This gene encodes a member of the semaphorin family, a group of proteins characterized by the presence of a conserved semaphorin (sema) domain. Whereas some semaphorins are transmembrane proteins, others are secreted. Semaphorins play a major role in axon guidance. The protein encoded by this gene may be involved in both peripheral and central nervous system development.
• Source: Mammalian cells
• Purity: > 95%, as determined by SDS-PAGE and visualized by silver stain.
• Molecular Mass: The mature recombinant human Semaphprin 6B/Fc chimera, generated by proteolytic removal of the signal peptide region, is a homodimeric protein. Based on N-terminal sequencing, the mature recombinant human Semaphorin 6B protein starts at Leu 26. The monomer of the recombinant human Semaphorin 6B has a calculated molecular mass of approximately 63.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 - 120 kDa protein in SDS-PAGE under reducing conditions.
• Activity: Measured by its ability to cause collapse of chick DRG growth cones. The ED50 for this effect is typically 2.5 - 5.0 μg/mL in the presence of 10 ng/mL of rhβ-NGF.
• Endotoxin: < 1.0 EU per 1 μg of the enzyme as determined by the LAL method.
• Format: Lyophilized from a 0.2 μm filtered solution in PBS.
• Storage: Samples are stable for up to six months at -70°C in a manual defrost freezer after the date of receipt. After receiving, this protein can be aliquoted and stored at above temperature for up to six months without a significant loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SEMA6B sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B [ Homo sapiens ]
• Synonyms: SEMA6B; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; SEMAN; semaZ; SEMA-VIB; SEM-SEMA-Y; SEMA6B; semaphorin Z; semaphorin VIB
• Gene ID: 10501
• mRNA Refseq: NM_032108
• Protein Refseq: NP_115484
• MIM: 608873
• UniProt ID: Q9H3T3
• Chromosome Location: 19p13
• Function: protein binding; protein binding; protein complex binding

Reviews

01/08/2022

The manufacturer offers a wide range of SEMA6B protein variants, including recombinant or native forms, as well as different conjugates and modifications.

04/26/2021

As a researcher using SEMA6B protein, I have found numerous advantages in its application during trials for various experimental purposes.

05/13/2017

The SEMA6B protein is known for its strong affinity for phospholipids, particularly phosphatidylserine (PS), on the outer leaflet of cell membranes.

Q&As

In which cancers is SEMA6B expression implicated?

SEMA6B has been associated with various cancers, including breast, lung, and prostate cancer, indicating its potential role in tumor progression.

How is SEMA6B expression regulated in normal physiological conditions?

The expression of SEMA6B is tightly regulated at the transcriptional and post-translational levels to ensure proper cellular functions.

Can SEMA6B be a potential therapeutic target?

Research suggests that targeting SEMA6B may have therapeutic implications, especially in conditions where axon guidance plays a role.

Are there any diseases associated with SEMA6B dysregulation?

Yes, altered expression of SEMA6B has been linked to certain neurological disorders and cancers.

How does SEMA6B contribute to axon guidance?

SEMA6B acts as a guidance cue for developing axons, influencing their growth and navigation during neural development.