Active Recombinant Mouse Sema6b Protein, Fc Chimera

• Cat.No.: Sema6b-987M
• Product Overview: Recombinant Mouse Sema6b (Accession # O54951), fused with , was produced in Human embryonic kidney cell, HEK293-derived.

Specification

• Source: HEK293 Cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Leu27
• Form: Lyophilized from a 0.2 µm filtered solution in Tris, NaCl and Tween® 20.
• Bio-activity: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.5 µg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta -NGF.
• Molecular Mass: Recombinant Mouse Sema6b, Fc Chimera has a calculated MW of 91 kDa (monomer). In SDS-PAGE migrates as 100-115 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Sema6b sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B [ Mus musculus ]
• Official Symbol: Sema6b
• Synonyms: SEMA6B; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; semaphorin-6B; sema N; sema VIB; semaphorin-N; semaphorin VIB; VIb; Sema; Seman; semaZ; MGC143995; MGC143996
• Gene ID: 20359
• mRNA Refseq: NM_001130456
• Protein Refseq: NP_001123928

Reviews

01/08/2022

The manufacturer offers a wide range of SEMA6B protein variants, including recombinant or native forms, as well as different conjugates and modifications.

04/26/2021

As a researcher using SEMA6B protein, I have found numerous advantages in its application during trials for various experimental purposes.

05/13/2017

The SEMA6B protein is known for its strong affinity for phospholipids, particularly phosphatidylserine (PS), on the outer leaflet of cell membranes.

Q&As

In which cancers is SEMA6B expression implicated?

SEMA6B has been associated with various cancers, including breast, lung, and prostate cancer, indicating its potential role in tumor progression.

How is SEMA6B expression regulated in normal physiological conditions?

The expression of SEMA6B is tightly regulated at the transcriptional and post-translational levels to ensure proper cellular functions.

Can SEMA6B be a potential therapeutic target?

Research suggests that targeting SEMA6B may have therapeutic implications, especially in conditions where axon guidance plays a role.

Are there any diseases associated with SEMA6B dysregulation?

Yes, altered expression of SEMA6B has been linked to certain neurological disorders and cancers.

How does SEMA6B contribute to axon guidance?

SEMA6B acts as a guidance cue for developing axons, influencing their growth and navigation during neural development.