Active Recombinant Human SPARC protein, His-tagged
Cat.No. : | SPARC-3193H |
Product Overview : | Recombinant Human SPARC protein(NP_003109.1)(Met1-Ile303), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1.5-7.5 μg/mL. |
Molecular Mass : | The secreted recombinant human SPARC consists of 297 amino acids with the predicted molecular mass of 34 kDa. As a result of glycosylation, rhSPARC migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Ile303 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 98 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | SPARC secreted protein, acidic, cysteine-rich (osteonectin) [ Homo sapiens ] |
Official Symbol : | SPARC |
Synonyms : | SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine; |
Gene ID : | 6678 |
mRNA Refseq : | NM_003118 |
Protein Refseq : | NP_003109 |
MIM : | 182120 |
UniProt ID : | P09486 |
Products Types
◆ Recombinant Protein | ||
SPARC-4111H | Recombinant Human SPARC Protein, His (Fc)-Avi-tagged | +Inquiry |
SPARC-2503H | Recombinant Human SPARC Protein, MYC/DDK-tagged | +Inquiry |
Sparc-6073M | Recombinant Mouse Sparc Protein, Myc/DDK-tagged | +Inquiry |
Sparc-010M | Recombinant Mouse Sparc Protein, His-tagged | +Inquiry |
SPARC-001H | Recombinant Human SPARC Protein, MBP-tagged | +Inquiry |
◆ Native Protein | ||
SPARC-287B | Native Bovine Osteonectin | +Inquiry |
SPARC-30653TH | Native Human SPARC | +Inquiry |
◆ Lysates | ||
SPARC-2120MCL | Recombinant Mouse SPARC cell lysate | +Inquiry |
SPARC-2616HCL | Recombinant Human SPARC cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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