Active Recombinant Human TEC(174-end) Protein, N-His-tagged

• Cat.No.: TEC-241H
• Product Overview: N-terminal 6Histagged, recombinant, human Tec amino acids 174–end expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose. Autoactivated by incubating with Mg/ATP, excess ATP and MgAc removed by gel filtration.

Specification

• Description: TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. It plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 174-end
• Form: 0.526mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.
• Bio-activity: 1400U/mg, where one unit of Tec, activated activity is defined as 1nmol phosphate incorporated into 400μM (EFPIYDFLPAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 57 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF LEEEDNSEEI VVAMYDFQAA EGHDLRLERG 61 QEYLILEKND VHWWRARDKY GNEGYIPSNY VTGKKSNNLD QYEWYCRNMN RSKAEQLLRS 121 EDKEGGFMVR DSSQPGLYTV SLYTKFGGEG SSGFRHYHIK ETTTSPKKYY LAEKHAFGSI 181 PEIIEYHKHN AAGLVTRLRY PVSVKGKNAP TTAGFSYEKW EINPSELTFM RELGSGLFGV 241 VRLGKWRAQY KVAIKAIREG AMCEEDFIEE AKVMMKLTHP KLVQLYGVCT QQKPIYIVTE 301 FMERGCLLNF LRQRQGHFSR DVLLSMCQDV CEGMEYLERN SFIHRDLAAR NCLVSEAGVV 361 KVSDFGMARY VLDDQYTSSS GAKFPVKWCP PEVFNYSRFS SKSDVWSFGV LMWEVFTEGR 421 MPFEKYTNYE VVTMVTRGHR LYQPKLASNY VYEVMLRCWQ EKPEGRPSFE DLLRTIDELV 481 ECEETFGR
• Purity: Purity 74.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TEC tec protein tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: TEC
• Synonyms: TEC; tec protein tyrosine kinase; PSCTK4
• Gene ID: 7006
• mRNA Refseq: NM_003215.3
• Protein Refseq: NP_003206.2
• MIM: 600583
• UniProt ID: P42680