| Description : |
Rse (TYRO3 protein tyrosine kinase), also known as Brt, BYK, Dtk, Etk3, Sky, Tif, or even sea-related receptor tyrosine kinase, is a receptor tyrosine kinase with homology to Axl. Rse is widely expressed in the adult and present in variable amounts in neural, lymphoid, vascular, and reproductive tissues. It plays a role in PI-3 kinase signaling and clearance of apoptotic cells. Gas6 acts directly on mature osteoclasts through activation of Tyro 3 and p42/p44 MAPK, possibly contributing to the bone loss by estrogen deficiency. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
451-end |
| Form : |
0.967mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
460U/mg, where one unit of Rse, active activity is defined as 1nmol phosphate incorporated into 250μM cdc2 substrate peptide per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
50.4 kDa |
| AA Sequence : |
1 MHHHHHHEFL RKRRKETRFG QAFDSVMARG EPAVHFRAAR SFNRERPERI EATLDSLGIS 61 DELKEKLEDV LIPEQQFTLG RMLGKGEFGS VREAQLKQED GSFVKVAVKM LKADIIASSD 121 IEEFLREAAC MKEFDHPHVA KLVGVSLRSR AKGRLPIPMV ILPFMKHGDL HAFLLASRIG 181 ENPFNLPLQT LIRFMVDIAC GMEYLSSRNF IHRDLAARNC MLAEDMTVCV ADFGLSRKIY 241 SGDYYRQGCA SKLPVKWLAL ESLADNLYTV QSDVWAFGVT MWEIMTRGQT PYAGIENAEI 301 YNYLIGGNRL KQPPECMEDV YDLMYQCWSA DPKQRPSFTC LRMELENILG QLSVLSASQD 361 PLYINIERAE EPTAGGSLEL PGRDQPYSGA GDGSGMGAVG GTPSDCRYIL TPGGLAEQPG 421 QAEHQPESPL NETQRLLLLQ QGLLPHSSC |
| Purity : |
Purity 89.6% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
