| Description : |
Wee1 is a protein kinase that plays an important role in the regulation of the cell cycle, particularly in the G2 checkpoint. It functions to inhibit the activity of cyclin-dependent kinase 1 (CDK1), a key regulator of the G2/M transition, by phosphorylating it on a specific tyrosine residue. This phosphorylation inactivates CDK1, preventing the cell from entering mitosis until DNA replication and repair processes have been completed. Research has suggested that Wee1 plays a role in several diseases, including cancer and neurodegenerative diseases. In cancer, inhibition of Wee1 has been suggested as a potential therapy to sensitize cancer cells to DNA damage and improve the efficacy of chemotherapy and radiotherapy. Additionally, Wee1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST,His |
| Protein length : |
214-end |
| Form : |
0.23mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
101U/mg, where one unit of Wee1, active activity is defined as 1nmol phosphate incorporated into 500μM (LSNLYHQGKFLQTFCGSPLYRRR) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
77 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MDTEKSGKRE 241 FDVRQTPQVN INPFTPDSLL LHSSGQCRRR KRTYWNDSCG EDMEASDYEL EDETRPAKRI 301 TITESNMKSR YTTEFHELEK IGSGEFGSVF KCVKRLDGCI YAIKRSKKPL AGSVDEQNAL 361 REVYAHAVLG QHSHVVRYFS AWAEDDHMLI QNEYCNGGSL ADAISENYRI MSYFKEAELK 421 DLLLQVGRGL RYIHSMSLVH MDIKPSNIFI SRTSIPNAAS EEGDEDDWAS NKVMFKIGDL 481 GHVTRISSPQ VEEGDSRFLA NEVLQENYTH LPKADIFALA LTVVCAAGAE PLPRNGDQWH 541 EIRQGRLPRI PQVLSQEFTE LLKVMIHPDP ERRPSAMALV KHSVLLSASR KSAEQLRIEL 601 NAEKFKNSLL QKELKKAQMA KAAAEERALF TDRMATRSTT QSNRTSRLIG KKMNRSVSLT 661 IYHHHHHH |
| Purity : |
Purity 86.3% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
