Recombinant Human WEE1 Protein (247-end), N-GST tagged

• Cat.No.: WEE1-35H
• Product Overview: Recombinant human WEE1 (amino acids 247-end) was expressed by E. coli cells using an N-terminal Glutathione-S-Transferase fusion protein.

Specification

• Description: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Liquid (sterile filtered)
• Protein Length: 247-end
• Purity: > 75 % by densitometry
• Applications: WEE1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. WEE1 Protein is suitable for use in Western Blot. Expect a band approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
• Stability: Expiration date is one year from date of receipt.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Shipping: Dry Ice

Gene Information

• Gene Name: WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 G2 checkpoint kinase; WEE1A; WEE1hu; wee1-like protein kinase; WEE1 homolog; WEE1+ homolog; protein kinase; wee1A kinase; EC 2.7.10.2
• Gene ID: 7465
• mRNA Refseq: NM_003390
• Protein Refseq: NP_003381
• MIM: 193525
• UniProt ID: P30291