Active Recombinant Mouse GPC3 protein, Fc-tagged

• Cat.No.: GPC3-1516M
• Product Overview: Recombinant Mouse GPC3 protein(Gln25-Met557), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human FGF basic, premium grade at 2 μg/mL (100 μL/well) can bind Mouse Glypican 3, Fc Tag with a linear range of 1-10 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 354-RQYR-357, and will be partially processed into N and C-terminal fragment with calculated MW of 38.0 kDa and 49.1 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln25-Met557
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gpc3 glypican 3 [ Mus musculus ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; glypican-3; OCI-5
• Gene ID: 14734
• mRNA Refseq: NM_016697
• Protein Refseq: NP_057906