| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
N/A |
| Form : |
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon Gamma at 2 μg/ml (100 μl/well) can bind Mouse IFNGR1 hFc, the EC50 of Mouse IFNGR1 hFc is7.0-30.0 ng/mL.2. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV).The ED50 for this effect is 0.05-0.3 ng/mL. |
| Molecular Mass : |
The recombinant mouse IFNG consists of 133 amino acids and predicts a molecular mass of 15.5 kDa. As a result of different glycosylation, it migrates as approximately 19.6 and 15.4 kDa band in SDS-PAGE under reducing conditions. |
| Protein Length : |
Met1-Cys155 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 85 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
