Active Recombinant Mouse Il12b Protein

• Cat.No.: Il12b-257I
• Product Overview: Recombinant Mouse Il12b Protein without tag was expressed in CHO.

Specification

• Description: Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
• Source: CHO
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 0.15 ng/mL, measured in a cell proliferation assay using 2D6 cells.
• Molecular Mass: 27-29 kDa (p35) and 45-50 kDa (p40), observed by reducing SDS-PAGE.
• AA Sequence: p35:RVIPVSGPARCLSQSRNLLKTTDDM VKTAREKLKHYSCTAEDIDHEDITR DQTSTLKTCLPLELHKNESCLATRE TSSTTRGSCLPPQKTSLMMTLCLGS IYEDLKMYQTEFQAINAALQNHNHQ QIILDKGMLVAIDELMQSLNHNGET LRQKPPVGEADPYRVKMKLCILLHA FSTRVVTINRVMGYLSSA
  p40:MWELEKDVYVVEVDWTPDAPGETVN LTCDTPEEDDITWTSDQRHGVIGSG KTLTITVKEFLDAGQYTCHKGGETL SHSHLLLHKKENGIWSTEILKNFKN KTFLKCEAPNYSGRFTCSWLVQRNM DLKFNIKSSSSSPDSRAVTCGMASL SAEKVTLDQRDYEKYSVSCQEDVTC PTAEETLPIELALEARQQNKYENYS TSFFIRDIIKPDPPKNLQMKPLKNS QVEVSWEYPDSWSTPHSYFSLKFFV RIQRKKEKMKETEEGCNQKGAFLVE KTSTEVQCKGGNVCVQAQDRYYNSS CSKWACVPCRVRS
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant murineInterleukin-12 (IL-12), remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, murine Interleukin-12 (IL-12), His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Il12b interleukin 12b [ Mus musculus ]
• Official Symbol: Il12b
• Synonyms: IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40
• Gene ID: 16160
• mRNA Refseq: NM_008352
• Protein Refseq: NP_032378
• UniProt ID: P43432