Active Recombinant Mouse Il15 Protein, His-tagged

• Cat.No.: Il15-7234M
• Product Overview: Recombinant mouse IL-15, His-tagged, was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

Specification

• Description: This gene encodes a a pleiotropic cytokine of the interleukin family of proteins that plays important roles in the innate and adaptive cell homeostasis, as well as peripheral immune function. The encoded protein undergoes proteolytic processing to generate a mature cytokine that stimulates the proliferation of natural killer cells. The transgenic mice overexpressing the encoded protein exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking the encoded protein exhibit impaired protection against a strain of attenuated Mycobacterium.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxicT cells. The ED50 for this effect is < 1 ng/mL.
  Activity Assay
  1. Cell line: CTLL2 (mouse cytotoxic T cell)
  2. Maintenance Condition: 10% FBS RPMI 1640 with hIL2
  3. Assay Medium: 10% FBS RPMI 1640 without hIL2
  4. Cell Density: 2 x 10^4 cells/well (96 well plate, final volume 100 μL)
  5. Starvation: 24 hr, Assay medium
  6. Incubation Time: 24hr (after sample treatment)
  7. Concentration Range: 0.039 ng/mL - 40 ng/mL
  8. Detection method: MTT assay
• Molecular Mass: 17.6 kDa
• AA Sequence: MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSHMNWIDVRYDLEKI ESLIQSIHIDTTLYTDSDFHPSCKV TAMNCFLLELQVILHEYSNMTLNET VRNVLYLANSTLSSNKNVAESGCKE CEELEEKTFTEFLQSFIRIVQMFIN TS
• Endotoxin: < 1.0 EU/μg of protein (determined by LAL method)
• Purity: > 90 % by SDS-PAGE
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: PBS, pH 7.4, containing 10 % glycerol

Gene Information

• Gene Name: Il15 interleukin 15 [ Mus musculus (house mouse) ]
• Official Symbol: Il15
• Synonyms: Il15; interleukin 15; IL-15; AI503618; interleukin-15
• Gene ID: 16168
• mRNA Refseq: NM_001254747
• Protein Refseq: NP_001241676
• UniProt ID: P48346

Reviews

04/19/2018

Mapping protein- interactions in DNA transcription for gene regulation.

02/20/2018

Suitable for cytotoxicity assays.

10/22/2017

Effective in NK cell activation.

Q&As

What techniques differentiate IL15 and IL2 receptor binding kinetics?

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases?

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions?

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation?

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells?

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses?

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays?

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion?

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR?

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation?

Flow cytometry quantifies IL15-driven T-cell proliferation.