Active Recombinant Mouse Il15 Protein, His-Tagged
Cat.No. : | Il15-01M |
Product Overview : | Recombinant mouse Il15 Protein, His-tagged was expressed in E. coli cell |
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Description : | Interleukin-15 (IL-15) is a cytokine with structural similarity to Interleukin-2 (IL-2). Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. |
Source : | Escherichia coli |
Species : | Mouse |
Tag : | His |
Form : | Lyophilized powder |
AA Sequence : | NWIDVRYDLEKIESLIQSIHIDTTL YTDSDFHPSCKVTAMNCFLLELQVI LHEYSNMTLNETVRNVLYLANSTLS SNKNVAESGCKECEELEEKTF TEFLQSFIRIVQMFINTS with polyhistidine tag at the N-terminus |
Endotoxin : | <0.1 EU per 1 μg of the protein by the LAL method. |
Bio-activity : | Measure by its ability to induce CTLL-2 cells proliferation. The ED50 for this effect is <10 ng/mL. The specific activity of recombinant mouse IL-15 is approximately >1x 10^5 IU/mg. |
Purity : | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
Storage Buffer : | The protein was lyophilized from a solution containing 1X PBS, pH 7.4. |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
Storage : | Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
Notes : | Please use within one month after protein reconstitution. |
Gene Name : | Il15 interleukin 15 [ Mus musculus (house mouse) ] |
Official Symbol : | Il15 |
Synonyms : | IL-15 |
Gene ID : | 16168 |
mRNA Refseq : | NM_001254747.2 |
Protein Refseq : | NP_001241676.1 |
UniProt ID : | P48346 |
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IL15-2683R | Recombinant Rat IL15 Protein, His (Fc)-Avi-tagged | +Inquiry |
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IL15-145H | Recombinant Active Human IL15 Protein, His-tagged(N-ter) | +Inquiry |
◆ Lysates | ||
IL15-5248HCL | Recombinant Human IL15 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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