Active Recombinant Mouse Il7 protein

• Cat.No.: Il7-767M
• Product Overview: Recombinant Mouse Il7 protein(NP_032397.1)(Glu26-Ile154) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: N/A
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically 0.4-4 ng/mL.
• Molecular Mass: The recombinant mouse IL7 consists of 130 amino acids and predicts a molecular mass of 15 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
• Protein Length: Glu26-Ile154
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il7 interleukin 7 [ Mus musculus ]
• Official Symbol: Il7
• Synonyms: IL7; interleukin 7; interleukin-7; Il-7; hlb368; A630026I06Rik; MGC129342
• Gene ID: 16196
• mRNA Refseq: NM_008371
• Protein Refseq: NP_032397