Active Recombinant Mouse Interleukin 15, His-tagged
Cat.No. : | Il15-533M |
Product Overview : | Recombinant Mouse IL-15 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer. |
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Cat. No. : | Il15-533M |
Description : | Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity and has structural similarity to IL-2 that is secreted by mononuclear phagocytes following infection by viruses. Stimulation by IL-15 requires interaction of Il-15 with components of IL-2R, including IL-2Rbeta and probably IL-2R gamma, but not IL-2R alpha. This cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. |
Concentration : | 0.5 mg/ml |
Source : | Mouse |
Host : | E.coli |
Form : | Supplied as a liquid in PBS, pH7.4, containing 10% glycerol. |
Purity : | > 90% by SDS - PAGE |
Sequence : | 49-162 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS |
Molecular Mass : | 17.6 kDa (152aa), confirmed by MALDI-TOF. |
Applications : | SDS-PAGE |
Endotoxin Levels : | < 1.0 EU per ug of protein (determined by LAL method) |
Bioactivity : | Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml. |
Storage : | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Gene Name : | Il15 interleukin 15 [ Mus musculus ] |
Official Symbol : | Il15 |
Synonyms : | Il15; interleukin 15; AI503618 |
Gene ID : | 16168 |
mRNA Refseq : | NM_008357 |
Protein Refseq : | NP_032383 |
UniProt ID : | P48346 |
Chromosome Location : | 8 C2; 8 38.0 cM |
Pathway : | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |
Function : | cytokine activity; cytokine receptor binding |
Products Types
◆ Lysates | ||
IL15-5248HCL | Recombinant Human IL15 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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