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Active Recombinant Mouse Interleukin 15, His-tagged

Cat.No. : Il15-533M
Product Overview : Recombinant Mouse IL-15 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
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Cat. No. : Il15-533M
Description : Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity and has structural similarity to IL-2 that is secreted by mononuclear phagocytes following infection by viruses. Stimulation by IL-15 requires interaction of Il-15 with components of IL-2R, including IL-2Rbeta and probably IL-2R gamma, but not IL-2R alpha. This cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
Concentration : 0.5 mg/ml
Source : Mouse
Host : E.coli
Form : Supplied as a liquid in PBS, pH7.4, containing 10% glycerol.
Purity : > 90% by SDS - PAGE
Sequence : 49-162 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
Molecular Mass : 17.6 kDa (152aa), confirmed by MALDI-TOF.
Applications : SDS-PAGE
Endotoxin Levels : < 1.0 EU per ug of protein (determined by LAL method)
Bioactivity : Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml.
Storage : Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Gene Name : Il15 interleukin 15 [ Mus musculus ]
Official Symbol : Il15
Synonyms : Il15; interleukin 15; AI503618
Gene ID : 16168
mRNA Refseq : NM_008357
Protein Refseq : NP_032383
UniProt ID : P48346
Chromosome Location : 8 C2; 8 38.0 cM
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Function : cytokine activity; cytokine receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques differentiate IL15 and IL2 receptor binding kinetics? 10/22/2022

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases? 06/23/2022

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions? 04/26/2022

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation? 09/19/2021

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells? 07/26/2021

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses? 02/04/2021

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays? 10/26/2019

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion? 02/03/2018

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR? 06/08/2017

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation? 05/26/2017

Flow cytometry quantifies IL15-driven T-cell proliferation.

Customer Reviews (3)

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Reviews
04/19/2018

    Mapping protein- interactions in DNA transcription for gene regulation.

    02/20/2018

      Suitable for cytotoxicity assays.

      10/22/2017

        Effective in NK cell activation.

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