Active Recombinant Mouse Ngf Protein (Ser122-Gly241), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Ngf-71M
• Product Overview: Recombinant Mouse Ngf Protein (Ser122-Gly241) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Beta-Nerve Growth Factors (Beta-NGF) is a 27 kDa cytokine with 241 amino acid residues. Beta-NGF belongs to neurotrophin family, and acts as neurotrophic factors. It's composed of alpha, beta, gamma subnuits, and the beta subunit is related to its biological activity. Beta-NGF binds to p75 neurotrophin receptor and Trk receptor and their function is about cell death and survival, respectively.
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant mouse beta-NGF is > 1 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 14.41 kDa.
  The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ser122-Gly241
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Ngf nerve growth factor [ Mus musculus (house mouse) ]
• Official Symbol: Ngf
• Synonyms: NGF; nerve growth factor; beta-nerve growth factor; beta-NGF; nerve growth factor, beta; Ngfb
• Gene ID: 18049
• mRNA Refseq: NM_001112698
• Protein Refseq: NP_001106168
• UniProt ID: P01139