Active Recombinant Mouse Trf protein, hFc-tagged

• Cat.No.: Trf-1958M
• Product Overview: Recombinant Mouse Trf protein(Q921I1)(Met1-His697), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 5-40 ng/mL.
• Molecular Mass: The recombinant mouse TRF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 919 amino acids and has a predicted molecular mass of 101.9 kDa. The apparent molecular mass of the protein is approximately 102 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Length: Met1-His697
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Trf transferrin [ Mus musculus ]
• Official Symbol: Trf
• Synonyms: TRF; transferrin; serotransferrin; siderophilin; beta-1 metal-binding globulin; hypotransferrinemia with hemochromatosis; HP; Tf; Tfn; hpx; Cd176; AI266983; MGC102653
• Gene ID: 22041
• mRNA Refseq: NM_133977
• Protein Refseq: NP_598738