Active Recombinant Rat Ace2 protein(Met1-Thr740), His-tagged

• Cat.No.: Ace2-1194R
• Product Overview: Recombinant Rat ACE2 (Q5EGZ1)(Met 1-Thr 740) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-His
• Protein length: Met1-Thr740
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH. The specific activity is >300 pmol/min/μg.
• Molecular Mass: The recombinant rat ACE2 comprises 734 amino acids and predicts a molecular mass of 85 kDa. The apparent molecular mass of the rat ACE2 is approximately 105-115 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ace2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Rattus norvegicus ]
• Official Symbol: Ace2
• Synonyms: ACE2; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme 2; renal angiotensin-converting enzyme 2; anigotensin-converting enzyme-related carboxypeptidase
• Gene ID: 302668
• mRNA Refseq: NM_001012006
• Protein Refseq: NP_001012006