Active Recombinant Rhesus IL10RA protein, hFc-tagged
Cat.No. : | IL10RA-981R |
Product Overview : | Recombinant Rhesus IL10RA protein(XP_001092736.1) (Thr9-Asn245) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus. |
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Source : | HEK293 |
Species : | Rhesus |
Tag : | hFc |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IL10RA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human IL10, The EC50 of biotinylated human IL10 is 39.0-92.0 ng/ml. 2. Measured by its ability to inhibit IL-10 dependent proliferation of MC/9 mouse mast cells. Approximately 0.1-0.4 μg/mL of IL-10Rα will inhibit 50% of the biological response induced by 10 ng/mL of recombinant human IL-10. |
Molecular Mass : | The recombinant rhesus IL10RA comprises 458 amino acids and has a calculated molecular mass of 51.8 KDa. |
Protein length : | Thr9-Asn245 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionSurface plasmon resonance and isothermal titration calorimetry techniques quantify binding kinetics between IL10RA and IL-10, revealing affinity and association/dissociation rates.
Cutting-edge flow cytometry and single-cell transcriptomics uncover the expression patterns of IL10RA across diverse immune cell subsets.
NMR spectroscopy captures dynamic interactions, revealing the flexibility and movements of IL10RA and IL-10 during their binding events.
Surface proteomics and mass spectrometry identify proteins physically interacting with IL10RA, providing insights into its complex functional networks.
Proximity labeling and quantitative proteomics unveil dynamic changes in IL10RA-associated proteins, shedding light on response mechanisms.
X-ray crystallography and cryo-electron microscopy provide detailed structural insights into the changes in IL10RA's 3D arrangement upon binding to IL-10.
Site-directed mutagenesis and alanine scanning pinpoint key amino acids in IL10RA essential for IL-10 interaction, aiding in understanding binding determinants.
Genome-wide CRISPR screening identifies genes and pathways affecting IL10RA expression and function, aiding in understanding its regulatory network.
Co-immunoprecipitation and FRET techniques elucidate the assembly of multi-protein IL10RA-IL-10 receptor complexes in cellular environments.
CRISPR/Cas9-modified cell lines and gene knockout models help dissect intricate signaling pathways activated by IL10RA upon ligand engagement.
Customer Reviews (3)
Write a reviewGood for receptor binding studies.
Consistent in bioactivity.
Suitable for ELISA.
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