Active Recombinant Streptococcus pyogenes Cas9 protein

• Cat.No.: Cas9 -121S
• Product Overview: Recombinant Streptococcus pyogenes Cas9 nuclease, was expressed with a N-terminal nuclear localization signal (NLS).

Specification

• Description: Recombinant Cas9 (10 µg/µl) is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease expressed with a N-terminal nuclear localization signal (NLS), provided in 50% glycerol. The Cas9 protein solution has been verified to be sterile and well-tolerated by mammalian cells when electroporated as a ribonucleoprotein complex (RNP) with a single guide RNA (sgRNA) for knockout experiments, or as an RNP with a donor repair template for knockin experiments.
• Species: Streptococcus pyogenes
• Bio-activity: 150 ng of Recombinant Cas9 (10 µg/µl) was tested in an in vitro cleavage experiment with a control sgRNA on a control PCR fragment. The Recombinant Cas9 (10 µg/µl), sgRNA, and PCR fragment were incubated at 37°C for 1 hr and a gel was run to verify that there was greater than 85% cleavage efficiency.
• Applications: Knockout experiments, or as an RNP with a donor repair template for knockin experiments
• Stability: 1 year from date of receipt under proper storage conditions.
• Storage: Store at –20°C
• Concentration: 10 µg/µl