Recombinant S. pyogenes Cas9 (D10A, H840A) Protein, His-tagged

• Cat.No.: Cas9-04S
• Product Overview: Recombinant full length SpCas9 (D10A, H840A)-NLS from S. pyogenes was expressed in E. coli using a C-terminal His tag.

Specification

• Description: Cas9 is a multidomain nuclease from the bacterium S. pyogenes characteristic of type II CRISPR- Cas systems. Using single guide RNA (sgRNA), Cas9 is targeted to specific genomic loci where it generates a blunt ended double strand beak. SpCas9 (D10A, H840A)-NLS is a catalytic mutant of Cas9 containing a nuclear localization sequence for optimal activity. Unlike wild type Cas9, SpCas9 (D10A, H840A)-NLS generates staggered double strand DNA breaks with overhangs. SpCas9 (D10A, H840A) can be used for various genome editing applications.
• Source: E. coli
• Species: S. pyogenes
• Tag: His
• Molecular Mass: Observed MW: ~160 kDa
  Calculated MW: ~161 kDa
• Purity: >80%
• Stability: One year at -70 centigrade from deta of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.
• Concentration: 0.2 μg/μL
  6.26 pmol/μg
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol.
• Shipping: On dry ice.

Gene Information

• Official Symbol: Cas9
• Synonyms: Cas9; Csn1; Spy_1046; Cas9 (D10A, H840A)-Nikase
• Protein Refseq: AAK33936
• UniProt ID: Q99ZW2